New at-line flow cytometric protocols for determining carotenoid content and cell viability during Rhodosporidium toruloides NCYC 921 batch growth
作者： Cláudia Freitas , Beatriz Nobre , Luísa Gouveia , José Roseiro , Alberto Reis
DOI: 10.1016/J.PROCBIO.2014.01.022
关键词:
摘要: Abstract Rhodosporidium toruloides NCYC 921 batch growth was monitored as a means to evaluate the yeast biomass potential source for production of carotenoids and other lipids. Carotenoid content, cell viability size were assessed by multiparameter flow cytometry. The saponifiable lipid fraction assayed gas–liquid chromatography. carotenoid increased during stationary phase, reaching 78 μg/g while total fatty acid content attained 32% (w/w) at end fermentation. profile suitable biodiesel purposes. As cells entered proportion with depolarised mitochondrial membrane permeabilised cytoplasmic increased, attaining 65% 14%, respectively. Nevertheless, high (82%) showed esterase activity. These results demonstrated that cytometry can be powerful at-line technique monitor growth, being useful process optimisation lab pilot scales.
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