Quality evaluation of Hypericum ascyron extract by two-dimensional high-performance liquid chromatography coupled with the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
作者： Xiu-Mei Li , Xue-Gang Luo , Chao-Zheng Zhang , Nan Wang , Tong-Cun Zhang
关键词:
摘要: In this paper, a heart-cutting two-dimensional high-performance liquid chromatography coupled with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was established for controlling quality of different batches Hypericum ascyron extract first time. comparison common one-dimensional fingerprint, second-dimensional fingerprint compiled additional spectral data and hence more informative. The H. further evaluated by similarity measures same results were achieved, correlation coefficients ten ＞0.99. Furthermore, we also antibacterial activity. result demonstrated that not significantly MTT. Finally, MTT powerful tool to characterize samples batch batch. Therefore proposed could be used comprehensively conduct control traditional Chinese medicines.
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