Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae

摘要: Membrane lipids act as solvents and functional cofactors for integral membrane proteins. The yeast plasma is unusual in that it may have a high lipid order, which coincides with low passive permeability small molecules slow lateral diffusion of Yet, proteins whose functions require altered conformation must flexibility within membranes. We determined the molecular composition located defined diameter model proteins, including APC-superfamily lysine transporter Lyp1. now use naturally surround Lyp1 to guide testing support normal functioning transporter, when reconstituted vesicles composition. find phosphatidylserine ergosterol are essential function, transport activity displays sigmoidal relationship concentration these lipids. Non-bilayer stimulate activity, but different types interchangeable. Remarkably, requires relatively fraction one or more unsaturated acyl chains. data predictions periprotein lipidome new narrow band immediately surrounding transmembrane stalk protein allows conformational changes protein.