Analysis of mexicanolide‐ and phragmalin‐type limonoids from Heynea trijuga using high‐performance liquid chromatography/electrospray tandem mass spectrometry

摘要: RATIONALE Limonoids, a class of tetranortriterpenoids, exhibit various biological effects, including acting as potent antifeedants and insect growth-regulators against pests. The analysis phragmalin- mexicanolide-type limonoids by collision-induced dissociation tandem mass spectrometry (CID-MS/MS) has not been reported. METHODS A high-performance liquid chromatography/electrospray ionization (HPLC/ESI)-MS/MS method was developed to investigate the fragmentation patterns [M+NH4]+ ions for nine reference limonoids. also used in identification limonoid compounds botanic extracts Heynea trijuga. RESULTS losses side chains furan part were major patterns. However, there variation relative abundances product resulting from same pathways. total 89 trijuga detected 50 these identified or tentatively characterized based on elemental constituents, pathways, profile compounds. In addition, isomers could be distinguished. CONCLUSIONS An HPLC/ESI-MS/MS simultaneously identify screen Copyright (c) 2013 John Wiley & Sons, Ltd.