Simultaneous separation and identification of limonoids from citrus using liquid chromatography-collision-induced dissociation mass spectra
作者： Guddadarangavvanahally K. Jayaprakasha , Deepak V. Dandekar , Shane E. Tichy , Bhimanagouda S. Patil
关键词: Glycoside 、 High-performance liquid chromatography 、 Mass spectrometry 、 Tandem mass spectrometry 、 Limonin 、 Chromatography 、 Mass spectrum 、 Collision-induced dissociation 、 Glucoside 、 Chemistry 、 Filtration and Separation 、 Analytical chemistry
摘要: Limonoids are considered as potential cancer chemopreventive agents and widely distributed in the Citrus genus aglycones glucosides. In present study, reversed-phase HPLC coupled with CID mass spectra was developed for simultaneous separation identification of glucosides limonoids from citrus. Five such limonin, deacetyl nomilin, ichangin, isolimonoic acid nomilin were identified by positive ion MS/MS, whereas five glucosides, viz. limonin glucoside, isoobacunoic obacunone nomilinic glucoside analyzed negative spectra. The method successfully applied to complex citrus samples seeds extracted methanol partially purified LC-CID achieved C-18 column; eight comparing retention times spectral fragmentation. To best our knowledge, this is first report on using technique.
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