Chapter 6 Method for Yeast Protein Synthesis in a Cell-Free System

摘要: Publisher Summary The chapter describes a yeast in vitro system analogous to the E. coli S-30 systems. This provides useful base for studies on transfer RNA (tRNA) function, inhibitors, ionic conditions, and supplementation with heterologous components, hopefully further development of system. Polyphenylalanine synthesis operates extremely high fidelity does not show some elongation defects other systems nor dependence Mg 2+ concentrations that can occur highly purified results from on, example, inhibitor mechanisms do always agree those obtained vivo or crude cell-free Thus have role confirming better resolved systems, particularly where confirmation is feasible. Yeast strain, growth harvesting breakage method, buffers, protective agents, freezing technique all critical requirements successful preparation an active