Specific, rapid, and sensitive enzymatic measurement of sphingomyelin, phosphatidylcholine and lysophosphatidylcholine in serum and lipid extracts

摘要: Abstract Objective Human serum sphingomyelin (SM) and phosphatidylcholine (PC) play important roles in the development of atherosclerosis. However, there are no rapid sensitive methods for SM PC measurement. The present report describes a novel enzymatic method measuring SM, lysophosphatidylcholine (lyso-PC) levels plasma lipid extracts. Design total choline-containing phospholipids (total PL), were measured using two-reagent system involving specific enzymes choline-based phospholipids. procedure was performed either microplate or automatic analyzer technology. concentration lyso-PC calculated by subtracting plus from PL concentration. Results Assay results showed linear correlations between sample absorbance. within-run between-run coefficients variation PC, concentrations 2.0–4.4% 0.9–2.9% analyzer. Analysis normal human that strongly correlated with ( r  = 0.9850). There moderate  = 0.6228)  = 0.7806). n  = 50) 0.54 ± 0.07, 1.99 ± 0.22 0.60 ± 0.15 mmol/L, respectively. Conclusion allowed rapid, simple, accurate measurement extracts serum. is suitable both assays.