The in vivo incorporation and metabolism of [1-C-14] linolenate (18 : 3n-3) in liver, brain and eyes of juveniles of rainbow trout Oncorhynchus mykiss L and gilthead sea bream Sparus aurata L.

摘要: Accumulation of docosahexaenoic acid (DHA; 22:6n-3) in brain and eyes during development has been demonstrated fish but it is not clear whether liver or neural tissues themselves are greater importance the biosynthesis DHA from dietary 18:3n-3. In present study, we investigated vivo metabolism intraperitoneally injected [1-14C]18:3n-3 liver, brains young juvenile fish. Metabolism was followed over a 48h time-course order to obtain dynamic information that could aid elucidation roles different provision The study performed both freshwater fish, rainbow trout Oncorhynchus mykiss L marine gilthead sea bream Sparus aurata determine effect low limitingΔ5-desaturase activity may have this process. As expected, results showed although incorporated more 18:3n-3 into its lipids, fatty by de saturation elongation generally trout. percentages radioactivity recovered tetraene pentaene products were than there no difference hexaenes. contrast, re covery significantly compared bream. species, percentage desaturated/elongated much lower eyes, increased time-course. though, highest desaturated eye observed after 12 24h, respectively. However 24-48h. Radioactivity 24:5n-3 24:6n-3, intermediates Δ4-independent ("Sprecher shunt") pathway for synthesis DHA, especially eyes. Overall, cannot eliminate role developing they suggest can be synthesised vivo.