Sequence analysis and insertional inactivation of a gene encoding Moraxella sp. CK-1 cell wall hydrolase.

摘要: Sequencing of the Moraxella sp. CK-1 autolysin (cell wall hydrolases) gene showed presence an open reading frame which encodes a polypeptide 273 amino acids with molecular mass 33,316 Da. A presumed ribosomal binding site, possible –10 and –35 region, rho-dependent terminators were found. The C-terminal region mature protein considerable homology Thermus serine proteinase. Enzyme assay suggests that recombinant has amidase or endopeptidase activity. Analysis peptidoglycan fragments, following treatment autolysin, indicates this is N-acetylmuramyl-L-alanine amidase. Insertional inactivation chromosome led to decrease in cell hydrolytic activity, clumping cells, color change. No lytic band present inactivated magA mutant by renaturing SDS-PAGE.