Constitutive Expression of Protective Antigen Gene of Bacillus anthracis in Escherichia coli

摘要: The fatal bacterial infection caused by inhalation of the Bacillus anthracis spores results from synthesis protein toxins-protective antigen (PA), lethal factor (LF), and edema (EF)--by bacterium. PA is target-cell binding common to two effector molecules, LF EF, which exert their toxic effects once they are translocated cytosol PA. major component vaccines against anthrax since it confers protective immunity. large-scale production recombinant protein-based requires overexpression protein. We have constitutively expressed in E. coli DH5alpha strain. found no increase degradation when plasmid instability was observed inside expressing cells. also scaled up expression bioprocess optimization using batch culture technique a fermentor. purified metal-chelate affinity chromatography. Approximately 125 mg (rPA) obtained per liter culture. It be biologically functionally fully active comparison anthracis. This first report constitutive gene coli.