Identification of Optimal Insertion Site in Recombinant Newcastle Disease Virus (rNDV) Vector Expressing Foreign Gene to Enhance Its Anti-Tumor Effect

摘要: Recombinant Newcastle disease virus (rNDV) is tumor selective and intrinsically oncolytic, which has been developed as a vector to express exogenous genes enhance its oncolytic efficacy. Our previous studies found that insertion sites of foreign gene in rNDV affected expression anti-tumor activities. However, the optimal site for remains unknown. In this study, we inserted enhanced green fluorescence protein (EGFP) IL2 into four different intergenic regions using reverse genetics technology. Recombinants rNDV-EGFPs rNDV-IL2s were successfully rescued, displayed similar growth kinetics with parental virus. Both EGFP mRNA levels most abundant HepG2 cells, when was between NP/P rNDV. Similarly, IL-2 expressed by cells infected rNDV-IL2 highest, site. To test whether these rNDVs higher could induce stronger response, treated H22-oxter-tumor-bearing C57BL/6J mice then examined The results showed rNDV-IL2-NP/P had strongest inhibition murine hepatoma carcinoma tumors. splenocytes isolated from reached highest degrees CD4+ T CD8+ cells. addition, animals' survival rate rNDV-IL2-NP/P-treated group than other groups. Taken together, demonstrate NP P junction rNDV's effects.