Combinations of Small RNA, RNA, and Degradome Sequencing Uncovers the Expression Pattern of microRNA⁻mRNA Pairs Adapting to Drought Stress in Leaf and Root of Dactylis glomerata L.

摘要: Drought stress is a global problem, and the lack of water key factor that leads to agricultural shortages. MicroRNAs play crucial role in plant drought response; however, microRNAs their targets involved response have not been well elucidated. In present study, we used Illumina platform (https://www.illumina.com/) combined data from miRNA, RNA, degradome sequencing explore drought- organ-specific miRNAs orchardgrass (Dactylis glomerata L.) leaf root. We aimed find potential miRNA⁻mRNA regulation patterns responding conditions. total, 519 (486 conserved 33 novel) were identified, which, 41 had significant differential expression among comparisons (p < 0.05). also identified 55,366 unigenes by RNA-Seq, where 12,535 differently expressed. Finally, our analysis revealed 5950 transcripts targeted 487 miRNAs. A correlation miRNA ata-miR164c-3p its target heat shock protein family (HSP70) member 5 gene comp59407_c0 (BIPE3) may be essential and/or adaptation orchardgrass. Additionally, Gene ontology (GO) Kyoto encyclopedia genes genomes (KEGG) analyses found "antigen processing presentation" was most enriched downregulated pathway Taken together, explored how they regulated. These results serve as valuable genetic resource for future studies focusing on plants adapted