In Vitro Antifungal Activity of Burkholderia gladioli pv. agaricicola against Some Phytopathogenic Fungi

作者: Hazem Elshafie , Ippolito Camele , Rocco Racioppi , Laura Scrano , Nicola Iacobellis

DOI: 10.3390/IJMS131216291

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摘要: The trend to search novel microbial natural biocides has recently been increasing in order avoid the environmental pollution from use of synthetic pesticides. Among these are bioactive secondary metabolites Burkholderia gladioli pv. agaricicola (Bga). aim this study is determine antifungal activity Bga strains against some phytopathogenic fungi. fungicidal tests were carried out using cultures and cell-free culture filtrates Botrytis cinerea, Aspergillus flavus, niger, Penicillium digitatum, expansum, Sclerotinia sclerotiorum Phytophthora cactorum. Results demonstrated that all tested exert studied fungi by producing diffusible which correlated with their ability produce extracellular hydrolytic enzymes. All significantly reduced growth bacterial cells more than filtrates. Bulkholderia produced volatile organic compounds (VOCs), inhibited fungal rate Fusarium oxysporum Rhizoctonia solani. GC/MS analysis VOCs emitted strain 11096 indicated presence a compound was identified as 1-methyl-4-(1-methylethenyl)-cyclohexene, liquid hydrocarbon classified cyclic terpene. This could be responsible for activity, also agreement work other authors.

参考文章(45)
Zafar Mehmood, Iqbal Ahmad, Faiz Mohammad, Shamim Ahmad, None, Indian Medicinal Plants: A Potential Source for Anticandidal Drugs Pharmaceutical Biology. ,vol. 37, pp. 237- 242 ,(1999) , 10.1076/PHBI.37.3.237.6296
Travis S. Walker, Harsh Pal Bais, Erich Grotewold, Jorge M. Vivanco, Root exudation and rhizosphere biology. Plant Physiology. ,vol. 132, pp. 44- 51 ,(2003) , 10.1104/PP.102.019661
Mohamed Chérif, Nicole Benhamou, Richard R. Bélanger, Occurrence of cellulose and chitin in the hyphal walls of Pythium ultimum: a comparative study with other plant pathogenic fungi Canadian Journal of Microbiology. ,vol. 39, pp. 213- 222 ,(1993) , 10.1139/M93-030
J. A. Zygadlo, C. A. Guzman, N. R. Grosso, Antifungal properties of the leaf oils of Tagetes minuta L. and T. filifolia Lag Journal of Essential Oil Research. ,vol. 6, pp. 617- 621 ,(1994) , 10.1080/10412905.1994.9699353
Shanmugam N. Prashanth, Giuliana Bianco, Tommaso R. I. Cataldi, Nicola S. Iacobellis, Acylhomoserine lactone production by bacteria associated with cultivated mushrooms. Journal of Agricultural and Food Chemistry. ,vol. 59, pp. 11461- 11472 ,(2011) , 10.1021/JF202313J
Dieter Haas, Geneviève Défago, Biological control of soil-borne pathogens by fluorescent pseudomonads Nature Reviews Microbiology. ,vol. 3, pp. 307- 319 ,(2005) , 10.1038/NRMICRO1129
Marco Kai, Uta Effmert, Gabriele Berg, Birgit Piechulla, Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen Rhizoctonia solani Archives of Microbiology. ,vol. 187, pp. 351- 360 ,(2007) , 10.1007/S00203-006-0199-0