Heterogeneity of dermal microvascular endothelial cell antigen expression and cytokine responsiveness in situ and in cell culture.

摘要: Microvascular endothelial cells (EC) recruit circulating leukocytes at sites of inflammation, in part through cytokine-regulated expression endothelial-leukocyte adhesion molecules. Adhesion molecule varies among vascular beds and EC within microvessels a particular bed. In the present study, we have examined patterns antigen cytokine responsiveness dermal microvascular (DMEC) skin organ culture model and, for comparison, cell culture. Within superficial plexus (SVP) normal skin, CD36 is undetectable on capillary loops expressed DMEC only 20% larger, horizontal vessels. not modulated by cytokines. Endothelial-leukocyte molecule-1 (ELAM-1) induced 6 24 h TNF or IL-1, restricted to venular side loop venules proper. Vascular (VCAM-1) inducible SVP TNF, IL-4, alone combination either time point. When inflamed culture, are responsive regarding VCAM-1 expression. deep (DVP). molecules all capillaries small Both ELAM-1 lesser extent, and/or IL-4 larger h. The vessels dermal-subcutaneous border were found be CD36-/ELAM-1+/VCAM-1+ after treatment. from 47 98% (mean 75%) seven separate isolates modified Upon IL-1 activation, 50 90% express persists high levels negligible both times. These results with differ human umbilical vein analyzed parallel, which completely CD36- show transient sustained response IL-1. summary, demonstrated that comprise heterogeneous population EC.