Protective activity of biflavanones from Garcinia kola against Plasmodium infection

摘要: Abstract Ethnopharmacological relevance Garcinia kola is a medicinal plant traditionally used for malaria therapy in Central Africa. Aim of the study To evaluate antimalarial potencies vitro and vivo pure biflavanones from G. kola. Materials methods The were obtained by bioassay-guided fractionation 70% ethanol extract seeds their chemical structures elucidated comparison NMR (1H 13C) mass spectral data with those provided literature. Plasmodium falciparum (FCR-3, cycloguanil-resistant strain Gambia) was assessments activities. Growth inhibition, intraerythrocytic development parasite morphology evaluated culture microscopic observation Giemsa-stained thin blood films. cytotoxicity compounds against KB 3-1 (human epidermoid carcinoma) cells MTT assay. In activities determined mice infected berghei (ANKA strain) following four-day suppressive test. Results an resulted isolation three (GB-1a, GB-1, GB-2) as its active principles. These displayed not only potent inhibitory activity P. proliferation but also potency through oral administration without signs acute toxicity. GB-1 found to exhibit strongest on IC50 0.16 μM, whereas it exhibited very low greater than 150 μM. During assay berghei, biological approximate ED50 100 mg/kg administration. shown increase average life span significantly compared that control (p Conclusions outcome GB-1a, GB-2 may be related traditional utilization this crude drug judging significant content nuts. showed most high selectivity index and, therefore could exploited identify molecular target, which subsequently helpful design novel therapeutics malaria. considered promising candidate trial using higher animals falciparum.