Isolation, characterization, and therapeutic activity of bergenin from marlberry ( Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats

摘要: Bergenin is one of the phytochemical constituents in marlberry (Ardisia colorata Roxb.) having antioxidant, anti-diabetic, and anti-inflammatory properties. A. has been used as an herbal medicine Southeast Asia particularly Northeast India to treat diabetes. was isolated from methanol extract leaf (MEACL) by column chromatography TLC profiling. Characterization structural validation bergenin were performed spectroscopic analyses. A LC-ESI-MS/MS method developed for quantitation validated per guidelines FDA EMA. The successfully utilized quantify concentration MEACL samples. Therapeutic efficacy investigated on streptozotocin-induced diabetic rats following standard protocols. supplementation significantly improved physiological metabolic processes turn reverses testicular dysfunction via increasing serum testosterone concentrations expression pattern PCNA, improving histopathological histomorphometric manifestations, modulating spermatogenic events germ cell proliferation, restoring sperm quality, reducing DNA damage, balancing antioxidant enzymes levels. Hence, alternate rich resources could be a therapeutic agent complications.