Perspectives for imaging mass spectrometry in the proteomics landscape.

摘要: A number of techniques are used in the field proteomics that can be combined to get most molecular information from a specific biological sample, fluid or tissue. Imaging often obtain local tissue samples. However, imaging experiments staining experiments, which rely on aspecific interactions between fluorescent markers and pre-defined (families of) peptide protein. Therefore, is as screening validation tool for presence proteins have been identified by other means. mass spectrometry (IMS) combines advantages MS microscopy single experiment. It technique does not require any labeling analytes provides high multiplexing capability with potential analyte identification. enables simultaneous detection potentially all peptides present at surface determination identification tissue-specific disease markers. The workflows IMS closely resemble those conventional proteomics. In this review, we describe step-by-step position evaluate role comparative landscape. We illustrate concise review true discovery oriented seamlessly integrates perceived either an alternative complementary technique.