Testosterone-induced micronuclei and increased nuclear division rate in L929 cell line expressing the androgen receptor.

摘要: Data on the possible genotoxic effects of testosterone are limited: in particular, potential clastogenic and/or aneugenic have not yet been properly investigated. An vitro micronucleus (MN) assay was performed L929 cells exposed to at doses 10, 15, 20, 30, and 40 μg/mL. Significantly increased MN frequencies were detected μg/mL after 24 h 48 incubation. The nuclear division index higher than A benchmark dose (BMD) calculation used estimate 1% extra risk level for tetranucleated cells. calculated 12.01 μg/mL, with a 95% lower confidence limit (BMDL) 8.98 μg/mL; corresponding BMD BMDL 17.35 10.69 respectively. 14.86 7.75 values 0.50 0.87μg/mL BMD. These findings suggest that intensity mitogenic effect increases upon prolonged exposure. results our study show acts both as agent