Effects of steroid hormones and antihormones in cultured cells.

摘要: Mouse L-929 fibroblasts contain androgen (AR), estrogen (ER) and glucocorticosteroid receptors (GR). The addition of androgens estrogens (10 to 50 nM) the culture medium stimulated cell growth this stimulation was inhibited when corresponding anti-hormones (cyproterone acetate or tamoxifen, 100 were present at same time. Glucocorticosteroids had negative effects on leading even death over 1 microM concentrations. a new antiglucocorticosteroid RU 486, glucocorticosteroids. 486 displays also anti-progesterone activity, but as cells have no progesterone receptor, only anti-glucocorticosteroid action compound studied. Cell adhesiveness plates increased by estrogen, decreased again, abolished these positive effects. All observations can be made in serum-free supplemented with insulin not other hormones, proteins factors. Cyproterone acetate, tamoxifen specific affinity for AR, ER GR, respectively. After incubation whole 3H-tamoxifen 3H-RU accumulation either estradiol receptor complexes nuclear fraction observed. A tamoxifen-resistant clone selected medium. This variant about 50% less than tam-sensitive antiestrogen binding sites which found particulate cells. Shionogi SC-115 mammary cancer AR ER. In presence testosterone (50 considerable difference Testosterone proliferation, anti-androgen cyproterone (100 completely stimulation. showed some antagonistic effect induced growth. shape cultured without different. testosterone, clonal observed contact inhibition, whereas absence hormone, formed monolayer. No time Both bound AR. murine thymoma lines W7TB T1M1b, is strong antagonist cytolytic response.(ABSTRACT TRUNCATED AT 400 WORDS)