Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection
作者： S. Loret , G. Nollevaux , R. Remacle , M. Klimek , I. Barakat
DOI: 10.1016/J.JCHROMB.2004.02.033
关键词:
摘要: Abstract We developed a treatment of urine samples allowing the analysis two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal indicator) and PEG 4000 (poorly indicator an abnormal increase permeability) by unique gel permeation chromatography (GPC) with refractometric detection. Urinary were extracted using mixed-bed resin composed C2 C18 layers. Permeability mean values determined in 11 human healthy subjects 24.20±9.30% 0.12±0.08% for, respectively, 4000. The percentage value to one corresponded index (IPI) 0.52±0.35 expressing low diffusion this poorly marker.
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