β-Mannoside mannohydrolase and the mobilization of the endosperm cell wall of lettuce seeds, cv. Grand Rapids.
作者： B. F. F. Ouellette , J. D. Bewley
DOI: 10.1007/BF00392128
关键词:
摘要: Mobilization of the endosperm cell-wall reserves Lactuca sativa L. cv. Grand Rapids requires endo-β-mannanase and α-galactosidase activity. A third enzyme, β-mannoside mannohydrolase (EC 3.2.1.25) is also involved. We have determined optimum extraction assay conditions for this which soluble only in high-salt (1 M NaCl) buffer. It located exclusively within cotyledons, association with a cellulosic fraction. Its substrates are products mobilization, mannobiose mannotriose, diffuse to cotyledons hydrolyzed extracellularly by mannohydrolase.
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