Development and evaluation of flow through assay for detection of antibodies against porcine cysticercosis.
作者： A. P. Kumar , M. Hafeez , K. V. Subramanyam , C. V. Rayulu , C. Sreedevi
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摘要: A flow through assay (FTA) was developed on cellulose acetate membrane for the serodiagnosis of porcine cysticercosis using cyst fluid (CFA) and whole antigens (WCA) Taenia solium metacestode. The consisted antigen T. metacestode coated onto membrane, mounted a flow-through test device to provide capture matrix. optimum concentration coating 250 ng. protein colloidal gold conjugate served as antigen-antibody detecting reagent. total 225 serum samples were tested two antigens. Results better with CFA (96.0% sensitivity; 96.0% specificity) compared WCA (92.0% specificity). also enzyme-linked immunosorbent assay. ELISA showed 96 per cent sensitivity both whereas; specificity 92 respectively. agrees closely those ELISA. cross-reaction observed in one out eight hydatidosis positive pigs (12.5%) by assays. highest diagnostic accuracy (96%) obtained CFA-FTA CFA-ELISA. For its high sporadic cross-reactions, appears be suitable practical use at field level without instrumentation.
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