Mechanistic Evaluation and Translational Signature of Gemcitabine-induced Chemoresistance by Quantitative Phosphoproteomics Analysis with iTRAQ Labeling Mass Spectrometry.

摘要: One of the main causations poor prognosis pancreatic cancer is lack effective chemotherapies. Gemcitabine a widely used chemotherapeutic drug, but limited therapeutic efficacy achieved due to chemoresistance. Recent studies demonstrated that presence stem cells may lead failure chemotherapy. Moreover, gemcitabine can promote stemness cells. We detected alterations in protein phosphorylation and signaling pathways after treatment using iTRAQ labeling LC-MS/MS, because it was featured with advantages strong separation ability analysis range. A total 232 differentially expressed phosphorylated proteins were identified this study. Gene Ontology revealed nuclear lumen, part organelle lumen enriched for cell components binding, poly (A) RNA binding molecular function. variety based on KEGG analysis. AMPK, mTOR PI3K/Akt verified exposure. we found there complex interactions modulating induced by exposure PPIs map. Our experiments identify potential targets strategies sensitizing gemcitabine.