Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila
作者： Jiawan Wang , Sheng Wang , Sheng Li , None
DOI: 10.1016/J.IBMB.2014.09.003
关键词:
摘要: The nuclear receptor complex for the insect steroid hormone, 20-hydroxyecdysone (20E), is a heterodimer of EcR and USP. It has been shown that Drosophila USP can be sumoylated in mammalian cells, but it unknown whether EcR-USP sumoylation naturally occurs Drosophila. In USP, not EcR, was by Smt3, only SUMO protein. presence enhanced sumoylation, which further 20E treatment. addition to Lys20 site, five potential acceptor lysine residues were predicted verified. Mutation sites or reduction smt3 expression RNAi attenuated 20E-induced reporter activity. Moreover, salivary glands, reducing decreased activity, gene expression, autolysosome formation. Importantly, at least partially, RNAi-mediated signaling resulted from protein levels conclusion, modulates maintaining
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