20-hydroxyecdysone upregulates Atg genes to induce autophagy in the Bombyx fat body
作者： Ling Tian , Li Ma , Enen Guo , Xiaojuan Deng , Sanyuan Ma
DOI: 10.4161/AUTO.24731
关键词: Molecular biology 、 Gene knockdown 、 Autophagy 、 RNA interference 、 Gene expression 、 Bombyx 、 Biology 、 Response element 、 Atg1 、 ATG8
摘要: Autophagy is finely regulated at multiple levels and plays crucial roles in development disease. In the fat body of silkworm, Bombyx mori, autophagy occurs Atg gene expression peaks during nonfeeding molting pupation stages when steroid hormone (20-hydroxyecdysone; 20E) high. Injection 20E into feeding larvae upregulated genes reduced TORC1 activity resulting induction body. Conversely, RNAi knockdown receptor partner (USP) or targeted overexpression a dominant negative mutant (EcR (DN) ) larval downregulated genes, confirming importance 20E-induction pupation. Moreover, vitro treatments with genes. Five were potentially primary-responsive, response element was identified Atg1 (ortholog human ULK1) promoter region. Furthermore, 4 key (namely Br-C, E74, HR3 βftz-F1) 20E-triggered transcriptional cascade to different levels. Taken together, we conclude that addition blocking for autophagosome initiation, upregulates induce
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