Effects of antimetabolites on the adhesion of an estuarine Vibrio sp. to polystyrene.

摘要: The effect of various metabolic inhibitors and antibiotics on the adhesion an estuarine bacterium, Vibrio proteolytica, to polystyrene was investigated. Cells were either exposed substratum antimetabolite simultaneously or grown in presence a 25% MIC presented absence antimetabolite. Based response elicited, these could be divided into three classes: (i) those that had little no (fluorodeoxyuridine nalidixic acid); (ii) only inhibited after growth at (ampicillin, oxacillin, streptomycin); (iii) attachment when administered with (azide, dinitrophenol, chloramphenicol, puromycin, azauridine, rifampin, p-chloromercuribenzoate, cephalothin). killed by heating, Formalin, mercuric chloride treatment also less adhesive than viable cells. Collectively, results indicate physiologically active cells are more dead impaired cells, impairment cell wall synthesis beta-lactam renders adhesive, energy production protein (including transcription) both involved some aspect process, whereas DNA is not.