Development and Application of Microsatellites in Carcinus maenas: Genetic Differentiation between Northern and Central Portuguese Populations

摘要: Carcinus maenas, the common shore crab of European coastal waters, has recently gained notoriety due to its globally invasive nature associated with drastic ecological and economic effects. The native ubiquity worldwide importance C. maenas resulted in it becoming one best-studied estuarine crustacean species globally. Accordingly, there is significant interest investigating population genetic structure this broadly distributed along invaded coastlines. Here, we developed polymerase chain reaction (PCR) primers for dinucleotide two trinucleotide microsatellite loci, resulting from an enrichment process based on Portuguese populations. Combining these three new markers six existing markers, examined levels diversity regions Northern Central Portugal. Genotypes showed that locus polymorphism ranged 10 42 alleles (N = 135) observed heterozygosity per 0.745 0.987 expected ranging 0.711 0.960; values typical marine decapods. revealed weak, but structuring among populations (global FST 0.004) across a 450 km (over-water distance) spatial scale. Combinations will be useful studying parameters at range scales throughout expanding range.