Effect of two conserved amino acid residues on DREB1A function.

摘要: Transcription factors of the DREBP subgroup and EREBP contain conserved DNA-binding domains called AP2/EREBP domains, which specifically bind to DRE cis-element GCC-box, respectively. The 14th 19th amino acid residues are absolutely in transcription as well subgroup. However, these two different from those To assess functional significance binding target sequence, Val (14th residue) Glu (19th domain DREB1A (a factor subgroup) were mutated individually or doubly Ala Asp, This made mutant identical corresponding Yeast vivo analysis showed that: 1) on a selective medium plate SD/His- Ura- Trp- + 30 mM approximately 60 3-AT, growth yeast cells containing HIS lacZ double reporter genes was normal transformation singly DREB1A, obviously inhibited seriously 14th/19th DREB1A; 2) quantitative assay beta-galactosidase activity that intensities expression decreased transformations types. experimental results revealed site mutation did not affect cis-element; their binding; sites binding. suggests (14th) (19th) crucial regulation cis-element.