Working Mechanism of Peroxiredoxins (Prxs) and Sulphiredoxin1 (Srx1) in Arabidopsis thaliana

摘要: Plants generate reactive oxygen species (ROS) as a by-product of normal aerobic metabolism or when exposed to variety stress conditions, which can cause widespread damage biological macromolecules. To protect themselves from oxidative stress, plant cells are equipped with wide range antioxidant proteins. However, the detailed reaction mechanisms these still unknown. Peroxiredoxins (Prxs) ubiquitous thiol-containing antioxidants that reduce hydrogen peroxide an N-terminal cysteine. The active-site cysteine peroxiredoxins is selectively oxidized sulfinic acid during catalysis, leads inactivation peroxidase activity. This oxidation was thought be irreversible. Recently identified small protein sulphiredoxin (Srx1), conserved in higher eukaryotes, reduces cysteine?sulphinic yeast peroxiredoxin. Srx1 highly induced by H₂O₂-treatment and deletion its gene causes decreased tolerance H₂O₂, suggest involvement oxidants. Moreover, required for heat shock functional, well conformational switch cytosolic peroxiredoxins. change enhances stability activity, indicating plays crucial role peroxiredoxin regulation mechanism. Thus, understanding molecular basis critical revealing mechanism action. We postulate here involved dealing via controlling recycling Arabidopsis. review article thus will describing functions Prxs Srx Arabidopsis thaliana. There special focus on possible interacting reducing hyperoxidized Cys-sulphenic Prxs.