Improving the diagnosis of bovine tuberculosis using gold nanoparticles conjugated with purified protein derivative: special regard to staphylococcal protein A and streptococcal protein G

摘要: Different ancillary immunodiagnostic tests were traditionally-established for diagnosis of bovine tuberculosis (BTB) either cellular or humoral as tuberculin skin test (TST), gamma interferon (INF-γ), and indirect enzyme-linked immunosorbent assay (iELISA). These had been consumed more time expensive, needed sophisticated equipment. To dissolve these problems, serological depending on immunity is the aim this work. Herein, slide-based agglutination was chosen a rapid simple field based purified protein derivative (PPD) antigen in addition to some supplementation materials such Staphylococcal A (SPA) Streptococcal G (SPG) improve detection BTB antibody serum samples. Gold nanoparticles (Au NPs) synthesized by ray, after complete characterization, Au NPs spherical, small-sized, stable without any impurities. Addition reagents serodiagnosis paramount important antibodies against (TB) it considered an easily possible way improving TB diagnosis. In work, 70 animals tested positive TST well 20 negative used immune response PPD slide using reporter regents (SPA and/or SPG) native recombinant. The density recorded read 4 degrees positivity with scores ranging from (-) very strong reaction (++++) occurred different times agglutination. Groups showed 100% reactivates employed Exp. 1, 2, 3 differentiation rated moderate (2+) (4+), predominant (3+). Pink-colored intensity associated strengthened reactions between PPD-conjugated each samples, which allows visual rapid, simple, effective attractive BTB. specificity sensitivity characterized. offers highest (83.6%) among other immunoassays, while lowest (57.4%). SPA/SPG best performance term combined (performance index) 175.4. Therefore, development uses reagent (such SPA co-agglutination (COAT), recombinant (rSPA/SPG) antigen, rSPA/SPG conjugate same also performed sensitive, specific, eco-friendly, low cost, shows great potential lab So, high reduction that yields similarly traditional techniques needed.