Empirical evaluation of non‐invasive capture–mark–recapture estimation of population size based on a single sampling session

摘要: Summary 1 Non-invasive genetic data analysed with capture–mark–recapture (CMR) models can be used to estimate population size, particularly for elusive and endangered species. Data generated from non-invasive sampling are different, however, conventional CMR because individuals contacted several times within a single session. Two methods have been proposed recently accommodate this type of data, but no study has attempted compare their estimates evaluate reliability compared independent size. 2 We investigated the accuracy estimating abundance lesser horseshoe bats Rhinolophus hipposideros by genotyping DNA droppings collected non-invasively at three colonies over 2 consecutive years. The number that each individual was ‘contacted’ (i.e. per individual) size two different published methods: maximum likelihood Bayesian estimator. 3 Among 586 samples extracted, 534 provided complete genotype six eight microsatellite loci, which enabled reliable discrimination 165 individuals. Statistical colony sizes often included obtained visual counts, validating method. Discrepancies appeared when capture heterogeneity not taken into account while it occurred. 4 Synthesis applications. We first step towards improving numbers demonstrating produced bat faecal high quality provide accurate even during only one Such protocols valuable management tools rare species in general. method is relatively easy cost-efficient session required.