Comparative evaluation of four genotyping methods for hepatitis C virus.

摘要: Four most widely accepted genotyping methods for hepatitis C virus (HCV) were applied to 40 HCV RNA isolates obtained from Slovenian patients in order determine the concordance and applicability of various systems. The four are: (i) amplification core region with genotype-specific primers; (ii) nested polymerase chain reaction (PCR) followed by hybridization type-specific probes; (iii) reverse line probe assay Inno LiPA (Innogenetics, Gent, Belgium) using probes 5' non-coding (NCR); (iv) restriction fragment length polymorphism analysis DNA amplified NCR. Additionally, discordant results nucleotide sequence a part NS-5 was performed. Both based on NCR found more sensitive than those region. None correctly classified all isolates. PCR primers identified as entirely unsuitable remaining could clearly differentiate between genotypes, but not reliable subtyping. specificity methods, which are or region, occasionally hampered, due lack excess variation their respective target regions.