Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells
作者： G.T. Lee , M.D. Price , C.A. Mejia , H.L. Galan , J.A. Arroyo
DOI: 10.1016/J.EJOGRB.2014.10.002
关键词:
摘要: Abstract Objectives To assess the concentrations of inositol and sorbitol, determine expression related osmolyte factors [nuclear factor activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); aldose reductase] in placentas pre-eclamptic (PE) patients trophoblast BeWo subjected to hypertonic stress vitro. Study design Control PE were collected. cultured a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, reductase ERK proteins. High-performance liquid chromatography used levels sorbitol cell lysates. Results Compared with control placentas, showed higher lower SLC5A3. Treated inositol, NFAT5 total protein, SLC5A3 reductase, increased activation compared cells. Conclusions Hyperosmolar conditions increase cells, may account levels. accomplish this through
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