Use of Hyperosmolar Stress to Measure Stress-Activated Protein Kinase Activation and Function in Human HTR Cells and Mouse Trophoblast Stem Cells

摘要: Embryo growth is inversely correlated with hyperosmolar stress-induced stress-activated protein kinase/jun kinase (SAPK/JNK) induction. To examine whether stress has similar effects in stem cells derived from the embryo, authors test trophoblast cells. The response of human placental and mouse cell lines are tested here. Peak phosphorylated SAPK/JNK was induced by 400 mM sorbitol at 0.5 hours. At this dose, there an SAPK/JNK-dependent decrease mitogenic, cMyc hours preceding cycle entrance 24 hours, decreases terminal deoxynucleotidyltransferase dUTP nick end labeling/apoptosis doses 50 to induces cJun prior SAPK/JNK-dependent, approximate 8-fold increase apoptosis mM. phosphorylation peaked 4 largely subsided 12 Thus, total exists before mediates rapid, homeostatic molecular responses that become biologic consequences after ends. This suggests continuity mechanisms functions lineage during implantation, which completely responsible for arrest apoptosis.