Oxygen levels that optimize TSC culture are identified by maximizing growth rates and minimizing stress.

摘要: Accumulating data suggest that 20% O(2) causes human and mouse placental trophoblast stem cell (TSC) differentiation suppresses proliferation. We tested the hypotheses phosphorylated stress-activated protein kinase (pSAPK) levels report optimal level for TSC culture, pSAPK responds to contradictory signals. dose range of 0-20% (0, 0.5, 2, 20%) on five effects in cultured TSC. The results showed 1) accumulation rates were highest at 2% O(2), lower lowest 0-0.5%; 2) higher 20%, 3) Cleaved caspase 3, an apoptosis marker, increased 0.5% was 0% O(2); 4) Three markers multipotency 2 significantly decreased 0.5%-0%; 5) In contrast three 0.5%-0%. Thus, is optimum as defined by growth rate markers, without appreciable apoptosis. addition, two lines evidence fibroblast factor (FGF)4 does not directly activate SAPK. SAPK activity increases transiently with FGF4 removal but decreases when switched from present. activated signals, either signal removed. Taken together, findings senses suboptimal signals during culture probably vivo.