Evidence for the Role of Horizontal Transfer in Generating pVT1, a Large Mosaic Conjugative Plasmid from the Clam Pathogen, Vibrio tapetis

摘要: The marine bacterium Vibrio tapetis is the causative agent of brown ring disease, which affects clam Ruditapes philippinarum and causes heavy economic losses in North Europe Eastern Asia. Further characterization V. isolates showed that all investigated strains harbored at least one large plasmid. We determined sequence 82,266 bp plasmid pVT1 from CECT4600T reference strain analyzed its genetic content. a mosaic closely related to several conjugative plasmids isolated vulnificus was shown be itself Vibrios. In addition, it contains DNA regions have similarity with other bacteria (Vibrio sp., Shewanella Listonella anguillarum Photobacterium profundum). number mobile elements, including twelve Insertion Sequences or inactivated IS genes an RS1 phage element CTXphi cholerae. organization underscores important role horizontal gene transfer through shuffling transposition events acquisition new resources generating modular organization. presents copy 9, relatively high for plasmid, appears belong type replicon, may specific Vibrionaceae Shewanelleacae.