An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import.

摘要: Nuclear import of simian virus SV40 large tumour antigen (T-ag) is enhanced by the protein kinase CK2 (CK2) site flanking nuclear localisation sequence (NLS). We report here that replacement this with a consensus for C (PK-C) can alter regulation T-ag and render it inducible phorbol ester. Measurement kinetics using fluorescently labelled proteins confocal laser scanning microscopy show introduced PK-C functional in enhancing compared to lacking site. Treatment activator 12-myristate 13-acetate (PMA) further increases level maximal accumulation initial rate. This engineered PMA-responsive NLS may have application targeting molecules interest nucleus response agents stimulating PK-C.