Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin.

摘要: Two restriction fragments from corynebacteriophage beta vir tox+ that encode peptides similar to diphtheria toxin fragment A and the chain termination fragment, CRM45, have been cloned into Escherichia coli in plasmid pBR322. Clones containing recombinant plasmids produced gene products were active catalyzing ADP ribosylation of elongation factor 2 reactive with antiserum. Toxin-related found primarily periplasmic compartment degraded nonimmunoreactive forms within 1 h synthesis. The expression both appears originated promoter.