Tracing Metabolic Changes in Soybean Cotyledons during Germination by NMR

摘要: The changes in 13C-NMR and 31P-NMR spectra 1H-NMR images soybean cotyledons during germination were investigated. Using 13C-NMR, fatty acid signals the form of triglycerides observed dry seeds, those approximately 18 days after start imbibition. Sucrose appeared at 16 hr disappeared 5 days. A signal was days, suggesting activation membrane metabolism cotyledons.31P-NMR 2 imbibition before any apparent change spectrum. peaks identified as sugar phosphate, inorganic phosphate cytoplasm vacuole, an unassigned compound, distinguishable vacuole-associated peak became prominent 31P-NMR.Distribution maps free water indicated that stored macro-molecular materials which bound consumed heterogeneously within cotyledon. relaxation time (T1) increased suddenly between ...