Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3',5'-monophosphate and tiazofurin.

摘要: : Novel antineoplastic agents, 8-chloroadenosine 3′,5′-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis. The aim this work was in vitro study either 8-Cl-cAMP or TR effects on B16/F10 B16/C3 mouse melanoma death. Significant inhibition obtained after application TR. presence number apoptotic cells evaluated using agarose gel electrophoresis flow cytometry. nuclei, treatment with did not significantly change cells, although it show a significant increase expression c-myc same results were 8-Cl-cAMP. level showed Concerning that analyzed agents exhibited other cancer further preclinical studies these drugs will potentially lead better understanding molecular their finally more efficient therapeutic approaches diseases.