Combined pulsed-Q dissociation and electron transfer dissociation for identification and quantification of iTRAQ-labeled phosphopeptides.
作者： Feng Yang , Si Wu , David L. Stenoien , Rui Zhao , Matthew E. Monroe
DOI: 10.1021/AC802605M
关键词:
摘要: Here, we report a new approach that integrates pulsed Q dissociation (PQD) and electron transfer (ETD) techniques for confident quantitative identification of iTRAQ-labeled phosphopeptides. The use isobaric tags relative absolute quantification enables high-throughput peptides via reporter ion signals in the low m/z range tandem mass spectra. PQD, form trap collision activated dissociation, allows detection mass-to-charge fragment ions, is especially useful sequencing contain post-translational modifications. Analysis phosphoproteome human fibroblast cells using sensitive linear spectrometer demonstrated this hybrid improves both ETD improved phosphopeptide identification, while PQD provides
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