Pathways in the development of liver macrophages: alternative precursors contained in populations of lymphocytes and bone-marrow cells.

摘要: The origin of dividing liver macrophages during states intense reticulo-endothelial stimulation has been studied in mice by means the T 6 marker chromosome. cells were isolated for cytological analysis Garvey’s technique collagenase and trypsin digestion. During proliferative phase graft-versus-host ( GVH ) reaction strain combination C 57BL → (C57BL x CBA-T6T6)F 1 , practically all mitosis donor karyotype, even when relatively pure suspensions thoracic duct small lymphocytes used as cells. Several lines evidence established that analysed part a macrophage response. had characteristics which reflected cell proliferation examined histological sections. This was largely restricted to sinusoids with phagocytic properties. same proportion these appeared be actively phagoctyic before their arrest metaphase Colcemid found normal mice. Furthermore, more than 70% sinusoidal incorporated 3 H -thymidine demonstrably and/or after labelling. Liver greatly depressed splenectomy 24 h injection cells, although karyotype still predominant. Similar techniques have applied syngeneic radiation chimaeras—( CBA CBA-T6T6 F ‘repopulated’ CBA- T6T6 bone marrow. When Corynebacterium parvum vaccine stimulant, two-thirds lymphocyte one-third or less derived from precursor marrow Using partial hepatectomy stimulate chimaeras, however, it overwhelming majority bone-marrow precursor. property proliferating combined colloid H-thymidine It is concluded either two different precursors populations recirculating respectively can proliferate preferentially, according nature stimulus. Evidence variety sources supports contention represents major source ‘normal’ macrophages. Whether amongst identifiable any previously recognized category not yet known. Its utilization only detected so far conditions stimulation.