Combined effects of nanoplastics and copper on the freshwater alga Raphidocelis subcapitata.

摘要: Nanoplastics are recognized as able to interact with other pollutants including heavy metals, and natural organic matter, implications for the potential risks biota. We investigated interaction of carboxylated polystyrene nanoparticles (PS–COOH NPs) copper (Cu) algal exudates (EPS) how such could affect Cu toxicity towards freshwater microalga Raphidocelis subcapitata. PS–COOH NPs behavior in presence EPS was determined by dynamic light scattering (DLS), while surface ions fluorimetric analysis. ICP-MS used test ion adsorption absence algae. The between cell wall assessed fluorescence microscopy. Short- long-term tests were carried out parallel assess impact on growth. Results showed altered nanoparticle charge hydrodynamic diameter following exposure, supporting hypothesis a protein corona formation. In contrast, no absorption observed NPs, either or No differences growth inhibition exposure only, combination short-term well tests. However, after 72 h PS-COOH walls appeared correspond morphological alterations, revealing disturbances mitotic cycle. Our findings confirm ability shown nanomaterials. Environmentally realistic scenarios thus needed evaluating nanoplastic toxicity, will not maintain their pristine nature once released into media. Prolonged use different end-points changes production seem more reliable investigation nanoplastic/algal interactions which can drive food chain transfer nanoplastics ultimately toxicity.