Synthetic studies on the inhibitory region of rabbit skeletal troponin I. Relationship of amino acid sequence to biological activity.

摘要: Twelve peptide analogs of the actomyosin ATPase inhibitory region rabbit skeletal troponin I (Tn-I) have been synthesized by solid phase method and tested for biological activity in both acto-S1(A1) systems. Acto-S1(A1) is a cleaner more facile system we found no important discrepancies results two These studies indicate that sequence 105 to 114 necessary inhibition on order reported 21-residue cyanogen bromide fragment Tn-I (residues 96 116). We shown importance lysine bulky side chain at position this inhibition. Both high peptides containing compared (45% molar basis) previously demonstrated tropomyosin specificity relative these applicable discussion Tn-I. proven actin concentration significant factor determining extent peptides. also established charges alpha-amino alpha-carboxyl groups peptide, which do not appear parent protein, can modify This must be taken into account when extrapolating from protein.