Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria
作者： Michael-Paul Robinson , Na Ke , Julie Lobstein , Cristen Peterson , Alana Szkodny
DOI: 10.1038/NCOMMS9072
关键词:
摘要: Current methods for producing immunoglobulin G (IgG) antibodies in engineered cells often require refolding steps or secretion across one or more biological membranes. Here, we …
nature.com
harvard.edu
sci-hub.se 参考文章(51)
Jeffrey V. Ravetch, Silvia Bolland, IGG FCRECEPTORS Annual Review of Immunology. ,vol. 19, pp. 275- 290 ,(2001) , 10.1146/ANNUREV.IMMUNOL.19.1.275
L. Jermutus, A. Honegger, F. Schwesinger, J. Hanes, A. Pluckthun, Tailoring in vitro evolution for protein affinity or stability Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 75- 80 ,(2001) , 10.1073/PNAS.98.1.75
Raphael Levy, Robert Weiss, Gang Chen, Brent L. Iverson, George Georgiou, Production of Correctly Folded Fab Antibody Fragment in the Cytoplasm of Escherichia coli trxB gor Mutants via the Coexpression of Molecular Chaperones Protein Expression and Purification. ,vol. 23, pp. 338- 347 ,(2001) , 10.1006/PREP.2001.1520
S. T. Jung, S. T. Reddy, T. H. Kang, M. J. Borrok, I. Sandlie, P. W. Tucker, G. Georgiou, Aglycosylated IgG variants expressed in bacteria that selectively bind FcγRI potentiate tumor cell killing by monocyte-dendritic cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 604- 609 ,(2010) , 10.1073/PNAS.0908590107
Dieter Palmberger, Dubravko Rendić, Philipp Tauber, Florian Krammer, Iain B.H. Wilson, Reingard Grabherr, Insect cells for antibody production: Evaluation of an efficient alternative Journal of Biotechnology. ,vol. 153, pp. 160- 166 ,(2011) , 10.1016/J.JBIOTEC.2011.02.009
Miro Venturi, Claudia Seifert, Carola Hunte, High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm Journal of Molecular Biology. ,vol. 315, pp. 1- 8 ,(2002) , 10.1006/JMBI.2001.5221
Yariv Mazor, Thomas Van Blarcom, Robert Mabry, Brent L Iverson, George Georgiou, Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli. Nature Biotechnology. ,vol. 25, pp. 563- 565 ,(2007) , 10.1038/NBT1296
S. L. Morrison, M. J. Johnson, L. A. Herzenberg, V. T. Oi, Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 6851- 6855 ,(1984) , 10.1073/PNAS.81.21.6851
Holger Koch, Nico Gräfe, Ralph Schiess, Andreas Plückthun, Direct Selection of Antibodies from Complex Libraries with the Protein Fragment Complementation Assay Journal of Molecular Biology. ,vol. 357, pp. 427- 441 ,(2006) , 10.1016/J.JMB.2005.12.043
Rahely Hakim, Itai Benhar, "Inclonals": IgGs and IgG-enzyme fusion proteins produced in an E. coli expression-refolding system. mAbs. ,vol. 1, pp. 281- 287 ,(2009) , 10.4161/MABS.1.3.8492