Expression and purification of the surface proteins from Andes virus.

摘要: Andes virus is the main causative agent of Hantavirus cardiopulmonary syndrome in South America. There are currently no vaccines or treatments against virus. However, there several evidences suggesting that antibodies envelope glycoproteins may be enough to confer full protection syndrome. The goal present work was express, purify and characterize extracellular domains Gn Gc. We generated two adenoviral vectors encoding Both molecules were expressed by transduction SiHa cells. found sGc ectodomain mainly secreted into culture medium, whereas sGn predominantly retained inside at very low concentrations (below 1 μg/mL). Treatment with proteasome inhibitor ALLN raised concentration cell but did not affect expression levels sGn. ectodomains purified immobilized metal ion affinity chromatography, recognized sera from persons previously exposed To our knowledge, this first addresses purification Our results demonstrate maintain epitopes on surface viral envelope. also highlights need explore new strategies achieve high-level these proteins for development a vaccine candidate