Microencapsulation of pancreatic islets. A technique and its application to culture and transplantation.

摘要: Summary Hamster pancreatic islets were encapsulated by a biocompatible membrane composed of the molecular sequence alginate-polylysine-alginate. The released insulin into culture medium in response to secretagogues short-term incubation. In long-term culture, maintained their insulin-releasing capacity for 28 days at level similar that unencapsulated islets. No overgrowth fibroblastic cells was observed inside capsule even after 70 culture. Further, hamster xenotransplanted streptozotocin-induced diabetic rats intraperitoneally. Some islets, which recovered from recipient 27 transplantation, found be viable, although prolonged normalization fasting plasma glucose levels recipients could not been achieved. On contrary, replaced massive connective tissue elements and insulin-positive B hardly detected within grafts 22 transplantation. results this study seem confirm potential application encapsulating technique primary parenchymal transplantation