Drosophila : methods and protocols

摘要: Methods in Molecular Biology Series Editor: John M. Walker Volume: Drosophila: and Protocols Edited by Christian Dahmann, Max Planck Institute of Cell Genetics, Dresden, Germany Contents Preface Contributors 1 Drosophila melanogaster the Development XX century Alfonso Martinez Arias 2 Getting started: An overview on raising using Hugo Stocker Peter Gallant 3 FlyBase: A database for research community Rachel Drysdale 4 The use P-element transposons to generate transgenic flies Andre Bachmann Elisabeth Knust 5 GAL4 system: a versatile system expression genes David A. Elliott Andrea H. Brand 6 mutagenesis Thomas Hummel Klambt 7 EMS Screens: From screening mapping Bokel 8 RNAi cultured cells Sandra Steinbrink Michael Boutros 9 homologous recombination Keith Maggert, Wei J Gong, Kent G. Golic 10 Recombinases their gene activation, inactivation, transgenesis Johannes Bischof Konrad Basler 11 Cuticle preparation embryos larvae Cyrille Alexandre 12 Immunolabelling H.- Arno J. Muller 13 Imaging cellular molecular dynamics live fluorescent proteins Matthieu Cavey Lecuit 14 Analysis neuromuscular junctions: histology vivo imaging Andreas Schmid Stephan Sigrist 15 imaginal discs Klein 16 pupal wing morphogenesis Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, SuzanneEaton 17 Microscopic analysis adult retina semi thin plastic sections Konstantin Gaengel Marek Mlodzik 18 Fluorescent situ hybridization protocols tissues Eric Lecuyer, Neela Parthasarathy, Henry Krause 19 Linear RNA amplification production microarray probes Ansgar Klebes B. Kornberg 20 identification, detection, functional microRNAs Nicholas Sokol 21 Extraction immunoblotting from Wodarz 22 Purification protein complexes mass spectrometry Christoph Juschke Jurgen Knoblich 23 Mass chromatographic purification native Natascha Kunert Alexander Brehm 24 Flow cytometric Aida Flor de la Cruz Bruce Edgar 25 cell lines as model systems an experimental tool Buzz Baum Lucy Cherbas Index