Immunopotentiation of Different Adjuvants on Humoral and Cellular Immune Responses Induced by HA1-2 Subunit Vaccines of H7N9 Influenza in Mice

摘要: In spring 2013, human infections with a novel avian influenza A (H7N9) virus were reported in China. The number of cases has increased over 200 mortalities to date. However, there is currently no vaccine available for the H7 subtype virus. Virus-specific cellular immune responses play critical role clearance during infection. this study, we undertook side-by-side evaluation two different adjuvants, Salmonella typhimurium flagellin (fliC) and polyethyleneimine (PEI), through intraperitoneal administration assess their effects on immunogenicity recombinant HA1-2 subunit H7N9 influenza. fusion protein HA1-2-fliC combined PEI could induce significantly higher HA1-2-specific IgG hemagglutination inhibition titers than alone at 12 days post-boost, superior specific group compared adjuvanted group. induced IgG1/IgG2a ratio numbers IFN-γ- IL-4-producing cells alone, suggesting mixed Th1/Th2-type response Th2 bias. Meanwhile, IgG2a IgG1 levels, which indicative profile. Consistent this, significant equal numbers, detected after vaccination. Moreover, marked increase CD69 expression proliferative index vaccines indicated that both candidates effectively antigen-specific responses. Taken together, our findings indicate elicit effective humoral responses, offering promise development successful