Flavones from Scutellaria baicalensis georgi attenuate apoptosis and protein oxidation in neuronal cell lines
作者： Joungil Choi , Craig C Conrad , Christina A Malakowsky , John M Talent , Chun-Su Yuan
DOI: 10.1016/S0304-4165(02)00217-9
关键词:
摘要: The oxidative modification of proteins plays a major role in number human diseases including Alzheimer's disease (AD). Flavones extracts Scutellaria baicalensis (SbE) have been reported to exceptional antioxidant properties. We examined the effects SbE on neuronal cells exposed stress. Neuronal HT-22 were low levels H(2)O(2) generated from glucose oxidase (GO) under conditions that caused cell death 24 h. mechanism was shown occur via apoptosis. Flavone (50 microg/ml) protected and increased viability 85+/-5% (P<0.001). flavones also content Bcl-2 cell, resulted its phosphorylation, contrast decreased Bax levels. Furthermore, oxidative-stress-induced protein carbonyl formation reduced nearly two-fold when pretreated with flavone extract. Two-dimensional electrophoresis (2-DE) showed less than 15% total visible oxidized oxidation specific for certain oxidation-sensitive proteins. These data support idea can attenuate oxidant stress protect lethal damage.
elsevier.com
sci-hub.se 参考文章(41)
A Amici, R L Levine, L Tsai, E R Stadtman, Conversion of Amino Acid Residues in Proteins and Amino Acid Homopolymers to Carbonyl Derivatives by Metal-catalyzed Oxidation Reactions Journal of Biological Chemistry. ,vol. 264, pp. 3341- 3346 ,(1989) , 10.1016/S0021-9258(18)94071-8
Yasushi Enokido, Hiroshi Hatanaka, Masashi Yamada, Takekazu Kubo, Takumi Satoh, Oxygen toxicity induces apoptosis in neuronal cells. Cellular and Molecular Neurobiology. ,vol. 18, pp. 649- 666 ,(1998) , 10.1023/A:1020633919115
Takumi Satoh, Yasushi Enokido, Takekazu Kubo, Masashi Yamada, Hiroshi Hatanaka, GRAM POSITIVE BACTERIA INDUCE IL-6 AND IL-8 PRODUCTION IN HUMAN ALVEOLAR MACROPHAGES AND EPITHELIAL CELLS Inflammation. ,vol. 23, pp. 217- 230 ,(1999) , 10.1023/A:1020269802315
Roger T Dean, Shanlin Fu, Roland Stocker, Michael J Davies, None, Biochemistry and pathology of radical-mediated protein oxidation Biochemical Journal. ,vol. 324, pp. 1- 18 ,(1997) , 10.1042/BJ3240001
A. A. Beg, D. Baltimore, An Essential Role for NF-κB in Preventing TNF-α-Induced Cell Death Science. ,vol. 274, pp. 782- 784 ,(1996) , 10.1126/SCIENCE.274.5288.782
U. Zabel, T. Henkel, M.S. Silva, P.A. Baeuerle, Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus The EMBO Journal. ,vol. 12, pp. 201- 211 ,(1993) , 10.1002/J.1460-2075.1993.TB05646.X
Christian Behl, John B. Davis, F. George Klier, David Schubert, Amyloid β peptide induces necrosis rather than apoptosis Brain Research. ,vol. 645, pp. 253- 264 ,(1994) , 10.1016/0006-8993(94)91659-4
Takahiko Ito, Xingming Deng, Boyd Carr, W. Stratford May, Bcl-2 Phosphorylation Required for Anti-apoptosis Function Journal of Biological Chemistry. ,vol. 272, pp. 11671- 11673 ,(1997) , 10.1074/JBC.272.18.11671
Craig C. Conrad, John M. Talent, Christina A. Malakowsky, Robert W. Gracy, Post-Electrophoretic Identification of Oxidized Proteins. Biological Procedures Online. ,vol. 2, pp. 39- 45 ,(1999) , 10.1251/BPO17
Thomas L Deckwerth, Jeffrey L Elliott, C.Michael Knudson, Eugene M Johnson, William D Snider, Stanley J Korsmeyer, BAX is required for neuronal death after trophic factor deprivation and during development Neuron. ,vol. 17, pp. 401- 411 ,(1996) , 10.1016/S0896-6273(00)80173-7